Aquaculture is expanding faster than any other animal food production industry but faces significant challenges, including environmental concerns such as eutrophication, and reliance on wild fish for feed production. Moreover, there is a growing consumer shift towards alternatives to conventional animal-sourced foods that involve minimal or no use of animals. Cultured fish fillets, produced through cellular agriculture, present a promising alternative that could address these challenges and influence aquaculture demand. The project CLEANFISH investigates the potential impact of introducing cultured fish fillets on the access to food, consumer acceptance and environmental sustainability within the seafood industry. As concerns over overfishing, habitat destruction, and food security intensify, cultured fillets offer a viable option to reduce reliance on traditional aquaculture. While initial consumer hesitancy and high production costs pose challenges, advancements in biotechnology and economies of scale may facilitate broader adoption. By applying Life Cycle Assessment (LCA), a comprehensive methodology that evaluates the environmental impacts of a product throughout its entire life cycle, to both recirculatory aquaculture systems (RAS) and cultured fish production, key environmental impacts and areas for improvement can be identified. This analysis aids in understanding how cultured fish could complement or compete with aquaculture, potentially leading to more sustainable seafood production systems and reshaping global seafood markets.