Background: the quality of the marine environment is essential for maintaining of public health. The marine environment may get contamination from sewage discharges from human- animal origin reach to the sea. Contaminants carried by sewage to the marine environment can also reach to the food chain of fish. Several Mediterranean countries are dumping their wastewater into the sea shores without treatment. Also, The Libyan Coasts are suffering from continues dumping of untreated sewage into their shores for many years, which may be leading to negative impacts on natural marine environment and introduce of zoonotic pathogens from the land to the sea. Microbiological pollution in the marine environment can occur. Methods: the sea cucumbers collected freshly from the Western Libyan Coast. They collected throughout different seasons. Bacteriological examination: After clinical examination, under complete aseptic condition, the sea cucumbers will be dissected to separate viscera and longitudinal muscle bands. Bacteriological swabs technique carried out for coelomic fluid, viscera of sea cucumbers. And spread onto enriched media under complete aseptic condition Tryptic soy agar (TSA) supplemented with 2 % NaCl and striking on selective media (Thiosulphate- citrate- bile salt- sucrose agar- TCBS) supplemented with 2% NaCl. Isolates identified by MALDI- TOF mass spectrometry. Antimicrobial resistance patters from the isolates of interest assessed with minimal inhibitory concentration (MIC) by broth dilution method. Virological analysis including COVID: The collected sea cucumbers will be desensitization with ice- immersion baths for 3 h and then dissect to extract the main internal organs: left and right respiratory tree, gonad, longitudinal muscle bands, anterior intestine and cloaca. Histopathological examination: The muscle, tube feet, intestine and gonads will be dissected out of the animal and fixed in 10% formalin. Determination of heavy metals: Body wall samples, at least 20g, collected for analysis of cadmium (Cd), copper (Cu), zinc (Zn) and lead (Pb). The samples will be analyzed by inductively coupled plasma mass spectrometer technique according to standard method UNI EN 15763:2010 and FDA Method 4.7 Version. Determination of microplastic: A portion of body wall tissue (20g) sampled without the use of any plastic materials and immediately frozen within aluminum foil. Microplastic extracted by solvents and chemically characterized. Nutritional characterization: A portion of single sea cucumbers body wall (about 5g d.w.) will be analyzed for proximate composition. A peculiar analysis of single fatty acids profile by gas chromatography carried out to assess potential differences of animals, FAs from different sites and eventually to identify lipid biomarkers. Moreover, sea cucumbers could effectively be used are bio indicators for monitoring the presence of heavy metals and other types of contamination in the marine environment of Western Coast of Libya. Thus, creating publishable data about the sea cucumbers that relating to estimating the pollution rates and heavy metal in Western Libyan coast will shape the future plane for effective control of such zoonotic pathogens with stop fishing from polluted areas. Also, monitoring of the occurrence of infectious diseases in both marine animals and humans can facilitate the detection of new infectious agents. Also, to find out how safe the Western Coast of Libya is for fish farming.