AQUA 2024

August 26 - 30, 2024

Copenhagen, Denmark

DIETARY SUPPLEMENTATION WITH Bacillus pumilus AND Bacillus subtilis IMPROVES PERFORMANCE, MICROBIOTA COMPOSITION AND FUNCTIONALITY IN WHITELEG SHRIMP Litopenaeus vannamei AND THE CULTURE WATER

Ana Rodiles1*, Mónica Juárez Ceballos2 , Florian Geay2, Eric Leclercq1, John W. Tinsley2

 

a Lallemand SAS, 19 rue des Briquetiers, 31700 Blagnac, France

b Biomar AS, Havnegata 9, 7010 Trondheim, Norway

*arodiles@lallemand.com

 



 Probiotics are frequently used in intensive shrimp farming  to  help secure or  enhance health and performance .  This study  assessed the effects of  in-feed supplementation with a Bacillus -based probiotic  on  shrimp  performance, composition and  inferred functionality  of the digestive microbiota with the view to screen , if any,  the microbial pathways manipulated.

The 8 -week  (56 days) grow-out trial used whiteleg shrimp ( 1.2 g) in clear water tanks  (290L tanks; 20 ppt; 27°C)  fed to apparent satiation .  Two treatments were applied in quadruplicate: a control group (CON ) fed a  non-supplemented diet (BioMAR) and a probiotic group (BAC ) fed the  same basal feed supplemented with  proprietary strains of Bacillus pumilus and Bacillus subtilis  at 6×106 CFU/Kg feed (50:50 by CFU ; Lallemand, France).  Microbiota  composition (16S rRNA V3-V4 region )  and functional inference (PICRUSt2 )  were analyzed in the digestive gland (HP) and  rearing  water  at the start and end of the trial.

D ietary treatments had a significant impact on  the HP bacterial composition . At  the end of the trial,  the  probiotic group  showed  a lower prevalence of Vibrionales and a higher prevalence of Bacillus genus as well as of  members of the Roseobacter clade (Phaeobacter , Ruegeria and Paracoccus) in particular . S hannon diversity  was  also  significantly higher  in the BAC group  and  this index was  significantly correlated with the  Rhodobacteraceae/Vibrionales (R/V)  ratio (r = 0.76) (Rodiles et al., 2022).  Functional inference  of the HP microbiota  revealed  645 KO differently abundant between groups . Of these,  96% were higher in the BAC compared to the CON group and  corresponded to the enhancement of  15 KEGG pathways  involved in vitamins and cofactors production, bile acid production, denitrification, fermentation and  of  serum  resistance to pathogens. Some effects on the water microbiota were documented and will be presented.

 In conclusion,  the probiotic-feed group exhibited a digestive microbiota with enhanced taxonomic and functional diversity. This diversity was associated with specific nutritional and health advantages, as inferred from their functional characteristics. The primary factor contributing to this diversity appears to be a decrease in the prevalence of the Vibrionales taxon due to probiotic feeding. Given that Vibrionales possess limited functional attributes, their reduced prevalence fosters a more diverse  and functional microbiota offering both nutritional and health benefits.

Rodiles et al .  Relationship between alpha diversity and Rhodobacteraceae/Vibrionales ratio as a potential biomarker of the intestinal microbiota of whiteleg shrimp Litopenaeus vannamei . World Aquaculture  May 24 - 27, 2022, Merida (Mexico).