AQUA 2024

August 26 - 30, 2024

Copenhagen, Denmark

EVALUATION OF THE MUCOSAL HEALTH OF ATLANTIC SALMON Salmo salar FED DIETS SUPPLEMENTED WITH BREWER’S YEAST ADDITIVES

 Sheu G. Odu-Onikosi*,  Noah E. Wood, Folasade D. Amulejoye,  Ben Eynon,  Glenn Harper, Matthew Emery, Holger Kühlwein , and Daniel L. Merrifield

School of Biological Sciences

University of Plymouth

Plymouth, PL4 8AA

sheu.odu-onikosi@plymouth.ac.uk

 



The Atlantic salmon (Salmo salar) is a prominent species in aquaculture, and optimizing its growth and health is of paramount importance to the aquaculture industry. Brewer’s yeast is a known source of essential nutrients and bioactive substances such as β-glucans, mannan-oligosaccharides, and nucleotides which can potentially enhance the growth and overall health of aquatic species. This study investigates the impact of incorporating two distinct commercial brewer’s yeast additives, an autolysed brewer’s yeast (ABY) and a soluble dried yeast extract (SDYE), manufactured by Leiber GmbH into the diet of Atlantic salmon juveniles on their performance and mucosal health.

 A 9 -week feeding trial was conducted in a cold freshwater recirculatory system  with Atlantic salmon juveniles (37.08g) at the University of Plymouth .  Three isonitrogenous and isocaloric  diets were formulated  to  meet the known  nutrient requirements of Atlantic salmon (Table 1). The control diet (T1) had no brewer’s yeast  additive  while the other two diets, T2 and T3, were supplemented with 0.25g/100 g of ABY  or SDYE, respectively. The fish (20 fish/70 L tank) were fed one of the three diets (n = 3 tanks ) at 1% of b iomass  per  day.  All treatments grew well with no significant difference among the treatments (data not shown) .

 At the end of the feeding trial,  skin and intestinal samples  were taken for intestinal assessments using light and electron microscopy , expression of immunoregulatory genes, haematology, and 16S rRNA metabarcoding.

 The result of the intestinal and skin histology revealed that the brewer’s yeast diets sign ificantly enhance (p<0.05 ) the intestinal physiology, and the goblet cell counts in the intestine as well as in the skin (Table 2).

Haematology result indicated that all treatments exhibited normal blood parameters with no significant difference in blood cell count and haemoglobin concentrations (data not shown).

 Ongoing analysis of expression of key immunoregulatory genes and a comprehensive 16S rRNA metabarcoding analysis of intestinal samples is being undertaken to identify the mechanisms which underpin improved mucosal data observed.