Vanamei shrimp is one of the shrimp commodities that is now popularly cultivated in Indonesia. However, as time goes by, vanamei shrimp are now susceptible to disease. One type of disease that often attacks vanamei shrimp is Acute Hepatopancreatic Necrosis Disease (AHPND). The use of herbal medicines for fisheries is now a new alternative to the use of chemical medicines, one of which is Purple Stepanot (Pseudocalymma alliaceum) leaves. Based on the technical data report for Ajos Sacha 2006, purple stepanot leaf simplicia (Pseudocalymma alliaceum) is effective as an immunostimulant because it contains sulfur compounds that are similar to those of garlic, which are dominated by alline and allyl sulfide compounds. This study aims to determine the antibacterial power of purple stepanot leaf simplicia against Vibrio parahaemolyticus bacteria and its effectiveness as an immunostimulant.
The methods used are the agar diffusion method (in vitro) and Bioassy (in vivo). The test results showed that purple stepanot leaf simplicia was antibacterial against Vibrio parahaemolyticus at all concentrations tested (100%, 50%, 25%, 12.5% and 6.25%. Application to vannamei shrimp shows that the compound content contained in purple Stepanot leaf simplicia is able to increase the body’s defense system of vannamei shrimp, which is characterized by an increase in the total immune parameters of hemocytes (THC) and phagocytic activity with the most effective and efficient dose being 6.25%