Various efforts have been made to prevent attacks by Vibrio sp bacteria, including the use of chemicals and antibiotics, which if used excessively will cause bacterial resistance. Another problem that arises is the risk of damage to the environment around farmed fish and human health, for this reason the use of these materials must be effective and efficient. The aim of the research was to determine the inhibitory power of antimicrobials when treating fish diseases in floating net cages and to determine the level of resistance to antimicrobial agents (Antimicrobial Resistance/AMR) to obtain recommendations for the continued use of antimicrobials. The disc diffusion test method with the antibiotics Oxytetracyine, Enrofloxacin and Tetracyline in the form of paper discs was used in the inhibition test. To identify bacteria, biochemical tests using bacterial culture media (TCBS) are used as well as biochemical tests using the API 20E kit. Inhibition of sensitivity in white sea bass with antibiotics oxytetracycline 60%, enrofloxacin 80%, tetracycline 60%, bullfin fish oxytetracycline sensitive 60%, enrofloxacin sensitive 100%, tetracycline sensitive 100%, tiger grouper oxytetracycline sensitive 60%, enrofloxacin sensitive 100% , tetracycline sensitive 60%, duck grouper sensitive 60%, enrofloxacin sensitive 80%, tetracycline sensitive 80% intermediate inhibition can be seen in sea bass intermediate 40%, enrofloxacin intermediate 0%, tetracycline intermediate 40%, oxytetracycline intermediate 40% , enrofloxacin intermediate 0%, tetracycline sensitive 0%, tiger grouper oxytetracycline intermediate 40% enrofloxacin intermediate 0%, tetracycline intermediate 40% while duck grouper oxytetracycline intermediate 40%, enrofloxacin intermediate 20%, tetracycline intermediate 20%, results of research on the use of antibiotics oxyitetracycline, endrofloxacin and tetracycline can be recommended for disease management in aquaculture, the results show that the inhibitory power of the use of the three types of antibiotics is capable of providing sensitive and intermediate responses to the bacteria Vibrio sp. For general bacteria, two types of bacteria have been found, V. parahaemolyticus and Vibrio alginolyticus