Diseases are the major biological challenges for sustainable growth of shrimp farming . The diseases like White Spot Syndrome, EHP and V ibriosis caused by Vibrio sp . are resulting in severe shrimp mortality and adverse economic impact in shrimp farming. The annual economic loss due to V ibriosis in Indian shrimp farms during 2018-19 was estimated to be 39.93 million USD. Unfortunately , antibiotics are the most widely used treatment measures against V ibriosis. The uncontrolled use of antibiotics may lead to increased risk of antibiotic residues and antibiotic resistant bacteria . This study aims to evaluate the dietary effect of organic acid-based product Selacid GG (0.1%, 0.2%, and 0.3%) in shrimp challenged with Vibrio sp. For each treatment, triplicate tank (30 shrimp/tank) were fed respective diets 7 days before and 7 days after Vibrio sp . challenge by immersion. At the end of experiment , all the S elacid diet fed group (0.1%, 0.2%, and 0.3%) showed significantly lower V ibrio load in hemolymph than the P ositive Control . Shrimp fed Sel 0.2% and Sel 0.3% diet group showed significantly lower Vibrio load in the hepatopancreas . Also, by qPCR all the S elacid diet fed group (0.1%, 0.2%, and 0.3%) showed significantly lower V ibrio load than the P ositive Control. Shrimp fed Sel 0.2% and Sel 0.3% group showed significantly higher activity of phenoloxidase enzyme. In total haemocyte count all the S elacid (0.1%, 0.2%, and 0.3%) diet fed group were showed significantly higher count. Whereas, i n growth parameters such as final body weight, weight gain, specific growth rate, and survival rate Sel 0.2% diet fed group had a significantly higher values than the P ositive Control group. Similarly i n histology, relatively less degeneration and necrosis of HP tubules and HP epithelial cells and inflammatory response such as haemocytic infiltration , encapsulation and granuloma were observed with Sel 0.2% diet fed group . Overall, the S elacid GG at 0.2% in the diet significantly reduces the Vibrio load and simultaneously improves the immunity, growth and survival of V ibrio challenged shrimp. Hence Selacid GG c an be used as the alternative dietary intervention against Vibriosis in shrimp aquaculture.