The emergence of disease in shrimp has governed much concern in bio security among shrimp farms with the recent reports on hepatopancreatic microsporidiosis (HPM) caused by Enterocytozoon hepatopenaei (EHP). The microsporidians present in shrimp remain a silent pathogen that prevents optimal shrimp growth. However, the biggest threat is in shrimp farm concerns, which is the primary focus in ensuring biosecurity. Hence, the objective of this report is to summarise the current knowledge of EHP and its infection in shrimp with technical guide . First, t his paper provides an prevention before stocking fry, quick analysis of the diagnostic methods for detecting EHP infections in shrimp aquaculture. Interventions with controlling biomass, average daily growth and sampling technique would be the second approach to addressing EHP diseases. The third approach is the using probiotics and organic acid for controlling biodiversification microorganisms and preventing Vibrio growth in the shrimp gut . Finally, a systematic guideline for shrimp pond and intervention is proposed. Thus, this study was aimed to shed light on effective methods for the prevention of EHP infection in shrimp. We also include application on enhancing innate immune biomolecules as future targets in the intervention strategies on the microsporidiosis life cycle in shrimp and its environment. Overall, this will result in reduced disease outbreaks in shrimp aquaculture, ensuring the shrimp production in pond by biosecurity and technical application in the future.