Vibrio parahaemolyticus poses a significant threat to shrimp farming, leading to substantial losses in the global shrimp industry over the past decade. This study investigates three probiotic trial methods aimed at assessing the efficacy of probiotic products in inhibiting V. parahaemolyticus in shrimp farming environments.
In Trial 1, a sewage water sample was obtained from central drainage and divided into tanks. 20 liter each tank. One tank served as the control without any probiotic product added, while others received 20 grams of various aerobic probiotic products. Aeration was conducted for 48 hours, and the green Vibrio count was tested before and after aeration to evaluate inhibition.
Trial 2 focused on inhibiting Vibrio parahaemolyticus in shrimp hepatopancreas and gastrointestinal tracts. Shrimp were fed with probiotic feed containing a 1% dosage of probiotic product for three consecutive days through auto-feeder feeding. Green Vibrio count tested before and after probiotic feed treatment.
In Trial 3, following the successful inhibition of V. parahaemolyticus in shrimp tissues in Tial2, the selected probiotic product was applied to fermented rice to cultivate two benefit groups: probiotic strains for enhancing biosecurity pond and copepods, a natural food source for postlarvae. The water stocking and zooplankton expansion procedure includes three steps: 24 hours of liquid expansion, 24 hours of wet expansion, and 5-7 days of expansion in the pond. These steps were initiated two weeks before seed stocking and continued for two weeks after seeding, once per week for a total of four applications. Green Vibrio count ( V. parahaemolyticus) levels in pond and shrimp tissues were monitored throughout the process.
The Comprehensive approach aims to assess the efficacy of probiotic products’ inhibition against V. parahaemolyticus in shrimp farming, contributing to enhanced biosecurity and productivity in the industry.