Alternative feeds are expected to respond to current feed problems, such as rising fish feed prices and pollution of the water environment due to the accumulation of feed residue. Black soldier fly larvae (BSFL), which may be used as an alternative to replace fish meal as feed ingredients . Beside that, supplementation of probiotics to BSFL substrates has shown beneficial effects, such as increasing the amount of lipid and protein content, feed efficiency, feed waste conversion while lowering the level of chitin and affecting the relative abundance of beneficial gut bacteria . A recent study reported that statistical analysis of the gut microbiome in BSFL showed that there are more than 11,000 bacteria, which indicates a high microbial abundance. The microbes indicated a high similarity across taxonomy orders and showed that probiotic supplementation in BSFL substrates affects the whole microbial community. The aim of this study is to investigate the effect of probiotic additions to BSFL substrates on their gut microbiome.
Larvae were 500 g of each treatment and placed into control or treatment containers. Larvae in control containers were fed daily with 250 g waste bread, while treatment containers were fed 250 g of waste bread supplemented with (approximately 1 x 10 7 g/diet) of Lactococcus JAL 37 and Bacillus PCP 1. After 7 days, DNA from larval guts were isolated and quantified by Wizard® Genomic DNA Purification Kit. The 16S rRNA gene were amplified apply for next-generation sequencing technology using Illumina Miseq PE250 sequencing platform (Illumina, United States).
The result showed in each treatment, there were 98 communal OTUs (26,50%) . The bacterial/OTU richness as indicated by Chao1 were ranged from 219.250 to 295.790. the evenness bacterial/OTU index as indicated by Shannon index was ranged from 3.300 to 3.357 . Whereas the bacterial/OUT dominance by Simpson’s index were ranged from 0.662 to 0.802. The microflora community composition of phyla and genera. The relative abundances of phyla were Bacteroidota, Proteobacteria, Fusobacteria, Firmicutes, Campylobacteria, Desulfobacteria, Actinobacteria was high. The relative abundances of genera Dysgomonas, Morganella, Fusobacterium, Bacteriodes, Sebaldella, Providencia, Phascolarctobaterium, Campylobacter, and Breznakia were high. These results indicate that probiotic addition on Black Soldier Fly larvae substrates affected on their gut microbiome community.