An eight- week feeding trial was conducted to evaluate the efficiency of a combination of novel feed ingredients in replacing fishmeal (FM) and also to assess their impact on growth and immune performances, a pparent d igestibility coefficient (ADC), antioxidant and digestive enzyme activities in Pacific white shrimp Penaeus vannamei. Five isonitrogenous ( Crude protein: 36.01%) and isolipidic ( Crude lipid: 7.5%) diets were formulated using different protein sources. Diet-1 (Control diet): used fishmeal as the main protein source ; Diet-2: fishmeal was replaced by poultry by-product meal(PBM), Broken rice (BR) and single cell protein (SCP) (1:1:1); Diet-3: fishmeal was replaced by insect meal(IM), rapeseed meal (RPM) and single cell protein (SCP) (1:1:1); Diet-4: fishmeal was replaced by fish waste(FW), peanut meal (PM) and single cell protein (SCP)(1:1:1); Diet-5, fishmeal was substituted by poultry by-product meal(PBM), Broken rice (BR) and single cell protein (SCP) insect meal(IM), rapeseed meal (RPM) (1:1:1:1:1:1:1). Juvenile shrimps with an average weight of 1 gram has been stocked in different experimental tanks fed with above diet following completely randomized design (CRD) in triplicates. At the end of the feeding trial, it was observed that Diet 5 had significantly improved (p<0.05) weight gain (13.55±0.34), and FCR (1.24±0.04) and recorded the best feeding efficiency when compared to other treatments. At the same time, diet 3 has shown an increase in weight gain (12.81±0.19) and SGR (4.55±0.07) than other diets. Diet 5 also showed significantly higher protease activity in both hepatopancreas and intestine tissues. Protein digestibility was significantly higher in diet 5. T he results of the present study demonstrated that a composite mixture of IM, RPM, and SCP (1:1:1) might be an effective substitution to replace fishmeal . In the meantime, a combination of PBM, BR, SCP, IM, RPM, PM, and FW (1:1:1:1:1:1:1) is an excellent alternative diet to replace fishmeal in shrimp feeds.