Marine bacterium Vibrio parahaemolyticus has been identified as an important agent of vibriois disease and acute hepatopancreatic necrosis disease in shrimp that can caused a big economic loss for shrimp farming. This bacterium also zoonotic pathogen which can cause gastroenteritis associated with the consumpsion of raw or undercooked seafood. This research aims to provide information related to the presence of V. parahaemolyticus in farmed shrimp, and was carried out as a step in ensuring the safety of fishery food products so that they are safe for consumption.
Isolation of V. parahaemolyticus is carried out by taking the hepatopancreas from five different shrimp from the same ponds and pooled. Briefly, the hepatopancreas was homogenized in tryptic soy broth with 2% NaCl medium. A loopful of culture was inoculated on CHROMagar and Vibrio TCBS agar and incubated at 37o C for 24 h. M auve colonies on CHROMagar and green colonies on TCBS agar were picked up and subjected to V. parahaemolyticus species-specific PCR targetting the toxR with amplicon size 369 bp. Antimicrobial susceptibility test was done using the disk diffusion method. Three antimicrobial agents were used are tetracycline (30 µg), oxytetracycline (30 µg) and enrofloxacine (5 µg). Interpretation of susceptibility test using the CLSI criteria.
A total of 73 shrimp samples were collected from different shrimp farm spread accross 10 districts in South Sulawesi , and 38 (52.05%) Vibrio isolates were confirmed as V. parahaemolyticus based on toxR gene-specific PCR. V. parahaemolyticus isolated showed high susceptibility to tetracyclin (100%), oxytetrcycline (100%), and enrofloxacine (92,1%). The result the result indicated that V. parahaemolyticus is widely prevalent in shrimp in South Sulawesi but have high susceptibility to antibiotics.