The study was conducted to investigate the prevalence of blood parasites, specifically Trypanosoma spp, in Nile tilapia (Oreochromis niloticus Linnaeus, 1758) from Zobe Reservoir, Katsina State. A total of 108 Nile tilapia from the four major landing sites of the reservoir, comprising male and female were purchased between July and September 2023 . T he fish were transported alive to the fish biology laboratory, Federal University Dutsin -Ma, for the blood parasites collection and examination. Sampled fish were measured for length and weighed. The study employed a microscopic examination of blood smears , to accurately identify and characterize t he Trypanosoma species present. Microscopic examination revealed the presence of Trypanosoma parasites in a significant proportion of the sampled fish. Blood parasites were identified and counted. Male samples had higher per cent of infestation (6.82%) than female samples (5.00%). The highest prevalence was recorded in samples location C while the lowest was recorded in sample location B. Experimental fish ( O. niloticus) samples obtained from samples location A were free from trypanosome, this could be due to the absence of leeches in the samples location. In conclusion, Oreochromis niloticus obtained from Zobe reservoir were infected by blood parasites (Trypanosoma spp ) that are of economic importance . The findings sugge st that the observed parasitic infections may badly affect in Nile tilapia populations in Zobe Reservoir.