In aquaculture, pathogen detection, diet optimization and selective breeding may employ Real time PCR and Targeted Next-Generation Sequencing (NGS) workflows. These conventional methods pose limitations on productivity and cost-effectiveness. The Biomar k TM X9, an advanced benchtop platform employing microfluidics technology, substantially elevates throughput in Real time PCR and NGS library preparation. With an impressive capacity to process up to 9216 reactions per run, it accommodates diverse assay configurations, including 96 samples against 96 targets and 192 samples against 24 targets. This feature significantly enhances scalability and cost-efficiency while necessitating minimal manual intervention. X9 offers ground-breaking prospects for the aquaculture industry, and we propose various workflows demonstrating how it can revolutionize the industry.
Aquaculture pathogen detection
X9 offers a unique capability to simultaneously analyze up to 96 aquaculture pathogens in a single run, representing a significant advancement over traditional real-time PCR methods limited to a multiplexing capacity of only 5 targets and typically require sequential testing based on initial findings.
The high throughput, multiplexing approach of X9’s comprehensive screening ability not only accelerates the process but also substantially reduces resource usage. Moreover, it dramatically lowers the cost per test, making it significantly more cost-effective than conventional qPCR methods .
Diet optimization
A specialized IFC PCR array can be designed to gain insights into the effects of diet and supplements on essential biological processes, thereby enhancing feed efficiency and growth. This array outperforms traditional RT-qPCR methods in efficiency, providing a swift means to evaluate how different diets, supplements, and environmental conditions impact fish culture.
Selective Breeding
SNP genotyping is widely utilized in animal breeding to identify specific traits. The X9 system, offering medium multiplexing (ranging from tens to hundreds of SNPs), ensures high throughput, exceptional call rates, and accuracy, at an affordable cost. This combination makes it an ideal choice for genotyping a vast number of samples in selective breeding programs.