The increase in shrimp production through aquaculture has been carried out thanks to the optimization of processes such as nutrition in the pre-maturation and reproduction phases of shrimp. In this work, the best culture and food system were proved during the prematuration phase to improve the reproductive characteristics of Litopenaeus vannamei males; The biofloc system was compared with clear water and an experimental pellet with a mixture of fresh food. Survival, sperm quality, immune system parameters such as prophenoloxidase (ProFO ) and hemocyte count, nutritional condition measured through metabolites in the hemolymph, hepatopancreas and reproductive tract, and oxidant stress biomarkers were measured. I n the hepatopancreas and reproductive tract and the relative expression of superoxide dismutase, catalase, glutathione peroxidase, ProFO , a2-macroglobulin and caspase genes in the reproductive tract.