Studies have suggested that the shrimp microbiota is related to the rearing water microbiome which is linked to disease outbreak. We at Keeton Industries have initiated a long-term study of the microbiomes of shrimp ponds to understand the population dynamics in healthy and diseased environments. We plan on using this information for probiotic management to improve water quality and shrimp health.
Previously, we reported the results of colony isolations from ponds that were either positive or negative for White Feces Syndrome (WFS) . We established a method that allowed us to collect the microbiome on filters and purify DNA of quality for PCR detection of Vibrio species, and genes associated with WFS .
In this study, we made colony counts (see Table) and isolations from 5 new ponds located in India with different intensity of WFS and the PCR studies were repeated. Results will be compared to the previous year and reported . In addition, Targeted Amplicon sequencing results will be reported for Samples A2 and A4.