Aquatic germplasm repositories can play a pivotal role in securing the genetic diversity of economically vital aquatic species. However, existing technologies for repository development and operation face challenges in terms of precision, efficiency, and cost-effectiveness, especially for microdevices used in gamete quality evaluation . In this study, we examined the potential of using 3-D stereolithography resin printing of microdevices to address these challenges and evaluated the capabilities and limitations of industrial-grade printers, consumer- level printers, and conventional microfabrication methods such as photolithography. To determine the capabilities of 3-D resin printers and evaluate differences in design versus printed features in a test object, the Integrated Geometry Sampler (IGS) and the Single-piece Sperm Counting Chamber (SSCC) were printed using an industrial 3-D resin printer (Profluidics 285D, CADworks 3D) with a cost of about $25K, high- pixel resolution of 28 µm and rapid printing speed, and a consumer-grade counterpart (Sonic Mighty 8K Phrozen) with a more affordable price around $400-$1000 , lower resolution and slower printing speed. The IGS design featured an array of negative and positive features, such as a semi-sphere, cones, and channels with dimensions ranging from 1 mm to 50 µm in width and depth. The SSCC consisted of grid and wall features to facilitate the counting of cells. The 3-D printed parts were compared with polydimethylsiloxane (PDMS) devices cast from a typical photoresist mold. The f abrication quality was evaluated by use of optical profilometry (Keyence , VR-6100 ) of parameters such as dimensional accuracy and surface morphology, as well as fabrication time and cost . The precision, reliability, and surface quality offered by industrial-grade 3-D resin printing were more than satisfactory for operations requiring features larger than 100 µm due to a very low discrepancy between actual size and mean of measured size in the range of 1 mm to 100 µm. Meanwhile, consumer-grade printers we re suitable for microdevices with features larger than 200 µm (Figure 1). These capabilities offer great promise for rapid development and widespread use of standardized microdevices for numerous applications, including gamete evaluation and “laboratory-on-a-chip ” applications in support of aquatic germplasm repositories.