This study evaluates the effect of L-ascorbyl-2-polyphosphate (Vitamin C) on Litopenaeus vannamei immune and antioxidant responses, as well as sperm quality of broodstock males. Four diets were formulated: Basal (control group ( 16 mg/kg of total Vitamin C), and the other three prepared with different vitamin C levels : A (322 mg/kg); B (628 mg/kg); and C (934 mg/kg) . Circulant cholesterol, triglyceride (TG), glucose, and total protein were measured. Activities and differential expressions of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and the immune gene system i n the reproductive tract were measured. Sperm quantity and quality were determined. The lowest activity of the antioxidant enzymes was obtained in B (628 mg/kg) and the highest one in C (934 mg/kg) (p ≤ 0.05) diets . The i mmune system genes only showed differences in hemocyanin where the highest expression was obtained in B (p ≤ 0.05) diet . The highest sperm quantity was determined in individuals fed B (p ≤ 0.05) diet, which supplemented with 628 mg/kg V itamin C benefit L. vannamei broodstock males because it guarantees less antioxidant system activity , suggesting a good physiological state.