Aquaculture America 2024

February 18 - 21, 2024

San Antonio, Texas

EFFECT OF L-ASCORBYL-2-POLYPHOSPHATE (VITAMIN C) ON IMMUNE AND ANTIOXIDANT RESPONSE OF Litopenaeus vannamei (BOONE, 1931) BROODSTOCK MALES

 Gabriela Gaxiota, Grecia Montalvo, Martin Arenas, Alvaro Barreto, Karla Escalante

 

mggc@ciencias.unam.mx

 



This study evaluates the effect of L-ascorbyl-2-polyphosphate (Vitamin C) on Litopenaeus vannamei immune and antioxidant responses, as well as sperm quality of broodstock males. Four diets were formulated: Basal (control group ( 16 mg/kg  of total Vitamin C), and the other three prepared with different vitamin C levels : A (322 mg/kg); B (628 mg/kg); and C (934 mg/kg) .  Circulant cholesterol, triglyceride (TG), glucose,  and  total protein were measured. Activities and differential expressions of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and  the immune gene system i n  the reproductive tract were measured. Sperm quantity and quality were determined. The lowest activity of the  antioxidant enzymes was obtained in B (628 mg/kg) and the highest one in C (934 mg/kg) (p 0.05) diets . The i mmune system genes only showed differences in hemocyanin where the highest expression was obtained in B (p 0.05) diet . The highest sperm quantity was determined in individuals fed B (p 0.05) diet, which  supplemented with 628 mg/kg V itamin C benefit  L. vannamei broodstock males because it guarantees less antioxidant system activity , suggesting a good physiological state.