Abstract: Dissolved oxygen is an important abiotic factor in aquatic ecosystems. To investigate the effects of acute hypoxic exposure on liver angiogenesis in largemouth bass, acute hypoxic exposure for 24 h was performed in this study. The results showed that the immunohistochemical analysis showed that hypoxia exposure promoted angiogenesis in the liver of largemouth bass. During 8h hypoxic exposure, the concentration and activity of vasodilator factors were significantly increased (P < 0.05). Hypoxia exposure promoted angiogenesis by upregulating the expression of MMP-2, jagged, AKT, PI3K and MAPK genes. On the contrary, the expression of anti-angiogenic genes was up-regulated at 8h (P < 0.05). Finally, genes and proteins that regulate angiogenesis show a clear temporal order. The expression of genes that promoted vascular sprouting and elongation was up-regulated within 4h to 8h of hypoxia exposure (P < 0.05). Genes that promote vascular maturation were highly expressed within 12-24 hours (P < 0.05). In conclusion, acute hypoxia promotes hepatic angiogenesis in largemouth bass via HIF-dependent pathways.