Aquaculture is the fastest growing sector that is meeting the global protein requirements as well as creating a stable earning source for many people. However, women’s contribution in aquaculture is immensely unrecognized, especially for safe fish production. Therefore, t his study investigates women’s contribution to aquaculture and their knowledge, attitude, and practices (KAP) on safe fish production in Mymensingh district of Bangladesh considering 120 randomly selected women. This study was conducted on polyculture fish production system . The KAP index was estimated to assess KAP level, and the Tobit model was used to determine the factors influencing KAP level of women. In addition, Structural Equation Model (SEM) was employed to determine the causal relationship among knowledge, attitude, and practice on safe fish production. Preliminary result s revealed that, women had significant contribution in fish production activities which was about 19.18% in terms of total person-days in a year. Among different fish farming activities, women contributed significantly (81.61%) to feed application during the culture period. Based on the KAP index, women’s level of knowledge and attitude on safe fish production were acceptable and good respectively, but practice level were not satisfactory. Furthermore, experience, training, farm ownership, and connection with pharmaceutical companies had statistically significant positive effects on the KAP level. On the contrary, education had insignificant effects on their KAP level. The results of SEM analysis found a significant positive association between women’s knowledge with practice and attitude level. Considering the findings, increasing level of knowledge of women can improve attitude and practice towards safer fish production . Hence, the government and NGOs should pay special attention to improving safe fish production knowledge, attitudes, and practices of women to recognize women’s contribution and enhance nutrition security for millions of fish-dependent households.