Low dissolved oxygen concentrations are often considered as a factor have been widely studied in influencing the performance of Litopenaeus vannamei as a common concern in aquaculture . Different studies with dissolved oxygen as a primarymain limiting factor, have indicated this is a has revealed valuable outcomesas a stressor factor thatwhich can influence on growth, tail flip speed, immune response , metabolic rates, and gene expression, but most research hasve been conducted in long period of hypoxia settings using water mixed with gaseous nitrogen and sodium sulfite to preserve hypoxia level . Hence, there is a However, there is a remaining gap in our to understanding of its effect in natural diurnal cycles and its impacts under natural production environments. Therefore, the objective of this study aimedwas to create low dissolved oxygen conditions in a green water system and its effect on survival rate, growth performance, apparent net prot ein retention, and metabolic enzymes in hemolymph. Additionally , tissues of gill and hepatopancreas were collected to search for inflammatory gene expression . Two trials were conducted a t Claude Peteet Mariculture Center i n Gulf Shores, AL. The first trial consisted of t hree levels of air supply (Low= 0.25, medium =0.35, high= 0.7 cubic feet per second (cfs)). It was set up with 12 tanks (750L) stocked at 35 shrimp/m2 and initial weight (1.99 ± 0.06g) maintaining an equal flow rate. The second trial consisted of two levels of aeration (Low = 0.25 and high= 0.7cfs) stocked at 30 shrimp/m 2 and initial weight (4.30 ± 0.24g) . Feed inputs were estimated with expected growth of 2 g week-1 for the first trial and 3 g week-1 for the second trial . Results showed that hypoxia environments were achieved in green water , constraining aeration levels (Figure 1). The levels of metabolic enzymes of the hemolymphhemolymph, such as Alanine aminotransferase and Alkaline phosphatase, were not significantly different among treatments in the first trial. Moreover, low dissolved oxygen concentration happened more frequently in higher biomass conditions, resulting in an increment 9 % of low dissolved oxygen occurrences in the second trial, affecting significantlysignificantly affecting survival (P=0.003) and growth (P=0.048).). Th erefore, growth performance is influenced by a reduction in density because of dissolved oxygen concentrations . WHAT IS THE CONCLUSIONlow DO produces larger shrimp ?