Aquaculture America 2024

February 18 - 21, 2024

San Antonio, Texas

PROTEOMIC PROFILING OF BCWD RESISTANT AND SUSCEPTIBLE RAINBOW TROUT LINES AND IDENTIFICATION OF NOVEL BIOMARKERS

Gregory D. Wiens* ,  David P. Marancik, Christopher C. Chadwick,

Ross M. Reid , Keira Osbourn  and Timothy D. Leeds

 USDA-ARS National Center for Cool and

Cold Water Aquaculture, Kearneysville, WV, 25430

greg.wiens@usda.gov

 



 At the U.S. National Center for Cool and Cold Water Aquaculture, we have selected a  line of rainbow trout with increased innate resistance against bacterial cold water disease  (BCWD)  caused by  Flavobacterium psychrophilum (Fp).  After five generations of sele ction, the resistant line (ARS-Fp -R)  exhibits  over 60 percentage points  higher survival compared to a reference susceptible line (ARS-Fp-S) .  To gain insight into the differential host response between genetic lines, we compared the plasma proteomes  on  day 6 following injection  challenge. Pooled plasma from unhandled , PBS-injected, and Fp -injected groups were simultaneously analyzed using  a  TMT  6-plex label  and 513 proteins were identified and compared .  Differentially abundant proteins included  tissue-damage proteins,  acute phase proteins, protease inhibitors and chemotactic factors .   Novel  ELISA and Spatial Proximity Analyte Reagent Capture Luminescence (SPARCLTM) assays were developed to confirm differential abundance .  In the susceptible line, a secreted C1q family member (designated complement C1q- like protein 3; C1q-LP3) was upregulated over 20-fold while only modestly upregulated, 1.8-fold, in the resistant line .  Skeletal muscle troponin C (STNC) , cathelcidin 2 (CATH2), haptoglobin, leptin, and growth and differentiation factor 15 (GDF-15) exhibited elevated concentration in susceptible-line plasma.  Complement factor h like-1 (CFHL-1) exhibited higher abundance in the resistant-line compared to the susceptible- line in both control and challenged fish and thus was a baseline differentiator between lines.  C1q-LP3 and STNC were elevated in Atlantic salmon plasma following experimental challenge with Fp . In summary, this study furthers the understanding of the differential host response to Fp and identifies salmonid biomarkers that may have use for genetic line evaluation and on-farm health monitoring.   

Acknowledgements:  This work was supported  by  the US Department of Agriculture, Agricultural Research Service [Project number 8082-32000-007 ].  Mention of trade names or commercial products is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S Department of Agriculture