Commercial production of marine fish juveniles still relies on the supply of live food, such as rotifers and Artemia . Pelleted diet substitution for live prey is important for reducing production costs and for sustaining a high and constant quality juveniles production in commercial hatcheries . The objective of this research is to test the potential of a novel commercial compound diet (INVE feed , INVE Aquaculture, Belgium) to replace and eliminate the use of rotifers and/or Artemia for live feeding of Florida Pompano . INVE Natura pRo (<100um & 100/250 um) and ExL (200/400 um & 300/600 um) are designed to reduce the need to use rotifers and Artemia by at least 50%. A study conducted by INVE in seabream ( Sparus aurata) using these feeds found increases in survival, wet weight, robustness, and decreases in malformations. To reach this objective, Florida Pompano eggs were supplied by Proaquatix (Vero Beach, FL). Eggs from the same batch were stocked at 26,000 eggs/liter in 16–110-gallon (416 L- filled to 330 liters) tanks at Live Advantage Bait LLC (LAB) and 30,000 eggs/liter in 16–200-gallon (757 L) tanks at Proaquatix . Hatching success was estimated at each farm (~72% at LAB and ~96 % at Proaquatix). A control group and 3 live feed replacement treatments (50% replacement for rotifers and Artemia , 80% replacement for rotifers and Artemia and 80% for A rtemia only) were tested in quadruplicate. Pre-trial eggs and larvae at 8, 18, 27 and 31 days post hatch (DPH) were sampled from each tank for fatty acid (FA), biometric, and microbiome analysis . The preliminary results showed that at 18 DPH (last day before introducing Otohime), the control treatment showed the highest weight gain followed by the 50% for rotifers and Artemia treatment (11.23 ± 2.25 mg and 7.39 ± 1.33 mg, respectively). At 29 DPH, the control also showed the highest weight gain, but within the standard deviation of the replacement treatments. At 18 and 29 DPH the control had the highest length , within the standard deviation of the replacement treatments with the largest difference in the 80% for Artemia only treatment (6.02 ± 1.21 vs. 4.71 ± 2.06 at 18DPH and 19.9 ± 1.13 vs. 18.13 ± 1.99 at 29 DPH, respectively. Fatty acid results showed a relatively similar profiles toward the end of the experiment between all treatments, but highest DHA content in the 80% for Artemia treatment at 18 DPH , with 4.92 ± 1.32% of total FAs in the control and 14.88 ± 2.86% of total FAs in the 80% for Artemia (Figure 1). We find the preliminary results very promising to replace live feeds by formulated diets, which will help sustain the production of stable high quality Florida pompano fingerlings. H owever, it is important to keep in mind that further nutrition experiments, to accurately determine larvae requirements is paramount , which will constitute a platform for formulating appropriate diet for larvae fish.