The present study was designed to evaluate certain hematological parameters of fresh water fish Labeo rohita in relation to the physiochemical properties of river and farm waters. For this purpose, the control group fish Labeo rohita collected from Fish Seed Hatchery Satiana Road Faisalabad, Pakistan. The experimental group fish was sampled from River Chenab, Chiniot , Pakistan. The physicochemical parameters like temperature, pH, dissolved oxygen, hardness and total dissolved solids were measured for both experimental and control groups . For hematological study the blood collected in Eppendorf tube with EDTA. Blood was drawn from the posterior caudal vein. Hematological analyses include RBC, WBC, PLT, HGB, HCT, MCV, MCH, MCHC, LYM, PW and PCT . The data was analyzed for significant results.
In case of morphometric parameters, weight of control fish was found higher 983.3±60.09 g than the weight of experimental fish 866.6±44.09 g. Length of control fish was found greater 7.1±0.33 inch than that of experimental fish 5.8±0.17 inch. Width of control was larger 3.1±0.05 than that of experimental was 3.06±0.08 inch. In case of physicochemical parameters of water like temperature of control fish water was higher 19.23±0.57 °C than that of experimental fish water 18.20±0.20 °C. pH of control fish water was lesser 7.54±0.27 than that of experimental fish water 8.64±0.29. DO of control fish water was higher 5.99±0.07 mg/L than that of experimental fish water 2.74±0.23 mg/L. Hardness of control fish water was less 74.70±3.30 mg/L than that of experimental fish water 143.8±4.41 mg/L. TDS of control fish water was lesser 979.3±27.45 mg/L than that of experimental fish water 1813.7±34.22 mg/L.
The red blood cell count of control and experiment was 0.90±0.057×109/L and 0.523±0.067×109/L (P<0.01). The white blood cell count of control and experiment was 16.76±0.622×109/Land 3.00 + 0.577 ×109/L (P<0.01). The hemoglobin content of control and experiment was 6.90±0.49 g/dL and 3.20±0.611 g/dL (P<0.01). The PCV % of control and experiment was 14.33 ± 0.881% and 18.20 ± 0.986% (P<0.05). The MCV content of control and experiment was 148.6±6.960fL and 119.3±6.359fL (P<0.05). The MCH (pg) of control and experiment was 76.96±2.530 and 62.66±1.283 pg (P<0.01). The MCHC of control and experiment was 51.70±0.814 g/dL and 53.13±1.848 g/dL (P>0.05). Similarly, the LYM count has significant difference between control and experiment (P<0.01). While PDW (%), MPV (fL) and PCT (%) have shown non-significant results (P>0.05).