Aquaculture America 2024

February 18 - 21, 2024

San Antonio, Texas

EVALUATION OF THE MUCOSAL HEALTH OF ATLANTIC SALMON Salmo salar FED TWO DIETARY YEAST ADDITIVES

 

 

 Sheu G. Odu-Onikosi*,  Noah E. Wood, Folasade D. Amulejoye,  Ben Eynon, Matthew Emery, Holger Kühlwein , and Daniel L. Merrifield

 

School of Biological Sciences

University of Plymouth

Plymouth, PL4 8AA

sheu.odu-onikosi@plymouth.ac.uk



The Atlantic salmon ( Salmo salar) is a prominent species in aquaculture, and optimizing its growth and health is of paramount importance to the aquaculture industry. Brewer’s yeast is a known source of essential nutrients and  bioactive substances such as β-glucans, mannan-oligosaccharides, and nucleotides which can potentially enhance the growth and overall health of aquatic species.  This study investigates the impact of incorporating two distinct  commercial brewer’s yeast additives , an autolysed brewer’s yeast (ABY) and a soluble dried yeast extract (SDYE), manufactured by Leiber GmbH into the diet of Atlantic salmon  juveniles  on their performance and mucosal health.

 A 9 -week feeding trial was conducted in a cold freshwater indoor recirculatory aquaculture system (RAS)  with Atlantic salmon juveniles (37.08g) at the University of Plymouth .  Three isonitrogenous and isocaloric  diets were formulated  to  meet the known  nutrient requirements of Atlantic salmon (Table 1). The control diet (T1) had no brewer’s yeast  additive  while the other two diets, T2 and T3, were supplemented with 0.25g/100 g of autolysed brewer’s yeast  or soluble dried yeast extract, respectively. The fish (20 fish/70 L tank) were fed one of the three diets (n = 3 tanks ) at 1 % of body weight  per  day.  Water quality parameters were monitored throughout the trial.  At the end of the feeding trial,  skin and intestinal samples  were taken for intestinal assessments using light and electron microscopy , expression of immunoregulatory genes, haematology, and 16S rRNA metabarcoding.

The result of the skin histology revealed that t here was a significant enhancement (p<0.05 )  of goblet cell abundance through dietary supplementation in the  fish fed T2 (18.22±2.48 /200µm) compared to the T1 (14.56±2.85 /200µm) and T3 (14.44±2.66 /200µm) groups.

 

 Ongoing analysis of histological parameters of the intestinal samples ,  expression of  key immunoregulatory genes ,  haematology, and a 16S rRNA metabarcoding analysis of intestinal samples.