Aquaculture America 2024

February 18 - 21, 2024

San Antonio, Texas

PYRIDOXINE REQUIREMENT OF PACIFIC WHITE SHRIMP Penaeus vannamei

 

Kyeong-Jun Lee*, Han-Se Kim  and Daehyun Ko

 

Department of Marine Life Sciences

Jeju National University

Jeju 63243,  South Korea

kjlee@jejunu.ac.kr

 



 Pyridoxine acts as a coenzyme in  many  reactions involved in  the nutrient metabolism . It  is an essential nutrient to maintain and enhance  the  growth performance, immunity and antioxidant capacity of  aquatic animals including  fish and shrimp. T his study  was  designed to  determine the optimal level pyridoxine  in diet for Pacific white shrimp.

A control (Con ) diet was formulated to  have  a low crude protein level (33%)  without pyridoxine supplementation. A negative control (Con-) diet was formulated with 0.4% tetracycline hydrochloride for the evaluation of the effects of pyridoxine synthesis by intestinal microorganism in  the shrimp. Six other diets were prepared by adding pyridoxine at 25, 50, 75, 100, 125 and 150 mg/ kg to Con diet (designated as P25, P50, P75, P100, P125 and P150, respectively). Each diet was randomly assigned quadruplicate groups of  each  20 shrimp (0.38±0.00 g) and fed for 45 days.

The growth performance was significantly increased by pyridoxine supplementation in diets. The d ietary pyridoxine improved the innate immunity and antioxidant capacity of shrimp. The highest activities of trypsin, chymotrypsin, lipase and amylase were observed in shrimp fed P75 diet.  Cell membrane  thickness  was  significantly increased in all the pyridoxine supplemented groups, except for P25 and P150, than in Con group.  Gene expression  was  significantly upregulated in cystathionine-β-synthase, cystathionine-γ-lyase, pyridoxal kinase and crustin up to P75 group. The broken line regression analysis indicated that t he optimum  pyridoxine  level of a low protein diet seems to be 75.7 mg/kg diet based on