Representative methods for inducing mutations include chemical treatment, radiation treatment, and genetic modification, and recent research on mutation has focused on genetic techniques. Among them, chemical mutagenesis substances have been studied in various ways, such as body color change by inducing mutations for the purpose of developing application technologies for the aquarium fish industry. In this study, three chemical mutagens were analyzed at a concentration of 2 mM. The zebrafish breeding water was immersed for 1 hour 3 times for 6 days at a concentration of 2 mM of each chemical mutagenic substance. And then after7 days, quantitative PCR were analyz ed using the b rain, liver and kidney . To obtain F1, after immersion, zebrafish were housed in a cage at a male/female ratio of 2:1, and F1 fertilized eggs were obtained 5 days later.
I nternal bleeding and fin damage were observed in colchicine and hydroxylamine, and severe organ damage was observed in colchicine. In gonad, a dead egg were observed in colchicine. As a result of comparative analysis after production of F1 individuals, there was no difference from the control group.
In the growth results from crosses between normal and mutant individuals, the best growth was observed in the cross between normal individuals, which was the control group. In hydroxylamine, the best growth was achieved in crosses between hydroxylamine male individuals and normal individuals, and it was confirmed that growth was fastest in that order: ENU and then colchicine.
As a result of observing changes in growth related genes in individuals immersed at a concentration of 2mM by qPCR, growth hormone receptor A (ghra) gene, a growth-related gene, was significantly different from the colchicine-treated experimental group in males, the control group, and the other chemical treatment group. On the other hand, females showed higher levels of hydroxylamine than females . The lysozyme gene, an immune-related gene, was highly expressed in colchicine in males, while the level in females was the same as in all chemical treatment groups. The nestin gene, a nerve-related gene, showed higher expression in all experimental groups compared to the control group in males, with the highest level in colchicine, and in females, it also showed the highest level in colchicine.
This Results related to immunity, neurons, and growth related to mutant substances have not been reported in many existing studies, and it is believed that the results of this study can be used as basic data. In addition, the results of this study were conducted on zebrafish that had previously been treated with mutagenic chemicals, and the mutational changes in F1 and F2 will later be compared and analyzed.