Aquaculture America 2023

February 23 - 26, 2023

New Orleans, Louisiana USA

RAPID, COST EFFECTIVE, SNP GENOTYPING USING STANDARD BIOTOOLS POWERFUL MICROFLUIDITICS TECHNOLOGY

Luke Stewart, Jamel Dobbs, Roberto Spada

 



The use of single nucleotide polymorphism (SNP) genotyping with non-model organisms—those whose genomes are yet to be sequenced—has increased in importance as it provides robust, comparative data sets that can be easily shared across organism communities for a variety of purposes. Non-model organisms have the added burden of low available genomic sequence information requiring custom SNP assay development. Until recently, SNP genotyping technologies have been prohibitive to these communities due to the high cost of developing and running quality SNP genotyping panels. Standard BioTools SNPtypeAssays and the X9 Real-Time PCR System have addressed these barriers with a custom assay design service, cost-effective and high qualitySNP assays, and a high-throughput workflow minimizing hands-on time. The salmon research community has been specifically hampered by the cost barriers, andwould benefit from the technology for conservation and management purposes. Using chum salmon (Oncorhynchus keta) as an example, we describe a simple workflow using Standard BioTools SNPtypeAssays, the X9 System, and 96.96 Dynamic Array™ Integrated Fluidic Circuits (IFCs) for Genotyping to achieve cost-effective and rapid development of a SNP genotyping panel. Moreover, SNPtypeAssays provide significant cost savings for high-throughput, routine testing post panel development.