Ipomea aquatica leaf contains high protein and carbohydrates and it is potentially a valuable feed for livestock. 45 days feeding trial was conducted to study the effect of different inclusion level of Ipomea aquatica leaf meal (IALM) based diet on the growth and gene expression of Insulin like Growth Factor Binding Protein-1 (IGFBP-1). The trial was conducted with one hundred eighty Labeo rohita fingerlings distributed into three different experimental groups including a control group in three replicates. Each group was fed with three different iso-nitrogenous and iso-energetic diets containing 10% (T1), 20% (T2) and 30% (T3) of IALM. Fishes were fed to satiation twice daily anddaily and at the end of the experimental period, Weight gain and Specific Growth Rate (SGR) was found to be higher in T1 group. Feed Conversion Ratio (FCR) was found lowest in group fed with 10 % IALM based diet. The total serum protein was recorded significant different (P<0.05) with highest values in T1 group. A relative expression study of IGFBP1 was carried out in the liver tissue by real time PCR. IGFBP-1 expression was recorded highest in T3 and the lowest was observed in T1 group. Overall, at 10% inclusion level Ipomea aquatica Leaf meal (IALM) showed growth performance when compared with control (33% replacement of De-oiled Rice Bran (DORB). The IGFBP-1 gene expression was inversely related with growth of fish. On the basis of observations, the study suggests that inclusion level of IALM more than 10% may have detrimental effects to growth and the results can be correlated with the growth performance of this important aquaculture species.