Botanical extract of Acacia nilotica pods have antifertility effects on Fish after administration for specific time. However, limited research has been done on evaluation of body composition and saponin content in muscles of Oreochromis shiranus fed on Acacia nilotica Pods extract, were by the estimation of the actual quantity of Saponins us secondary metabolites, anti-nutrition factor and the influence on organoleptic analysis to further consumption from human being.. Because of that, experimental trial was conducted at Bunda fish farm in order to evaluate body composition and safety of Oreochromis shiranus (O. shiranus) fed on A. nilotica pods extract. Male O. shiranus with mean weight of 100±0.09g was stocked at rate of 15 fish/tank, for 18 weeks. The treatments were completely randomly assigned in triplicate in the experimental units (Control Feed 0gkg-1of the inclusion level of A. nilotica extract and Feed with A. nilotica extract at inclusion level of 15g/kg). The data was transferred from excel to R statistical software, for analysis, were T-Tests for independent sample and one way Analysis of Variance (ANOVA), at statistical significance level set at P<(0.05), after that, Tukey test was performed. According to the standard methods, the results indicated that the Acacia nilotica pod extract have Phytochemical such as Saponins, Tannins, Terpenoids, Antraquinones and Anthocyanins; about the residual bioactive compound (Saponins) there were statistical difference at P<(0.05); at termination point the Saponins(%) was (5.025±0.591) and it was reducing up to (1.761±0.030) it in 4 weeks period after termination the administration of the A. nilotica in the fed. Body Composition there were statistical difference at P<(0.05), Protein (70.125±0.554)- (53.330±3.095), Ash (24.389±1.411)- (16.131±0.617) termination point was superior than the Control. In terms of Fat there were statistical difference at P<(0.05), however control presented more than the termination point (14.265±0.0142)-( 9.198±0.823), on the organoleptic there were not statistically significantly different from zero at P>(0.05). The results of this study suggest that at the inclusion level of the 15 g/kg O. shiranus can be safer for consumption in 2 weeks after termination of the extract administration, since the amount of crude saponins is lower than the toxic level.