Agriculture technology (AgTech) refers to soilless plant production encompassing hydroponics (using synthetic fertilizer) and aquaponics (using fish waste fertilizer) that aims to improve yield, efficacy, and profitability by incorporating high-tech applications. Soilless agriculture has several advantages over soil-based agriculture such as efficient nutrient regulation and higher planting density resulting in higher yield per hectare along with better quality produce. In this project, we focus on utilizing fish feed as well as synthetic fertilizer (for comparison) as nutrients for growing bib lettuce (Lactuca sativa var. capitata), tomato etc. Nutrient uptake and distribution of these nutrients through fish, bacteria, and plants will be investigated for identifying important nutrients and their ratios for higher crop productivity. Epigenetic changes due to nutrient source (synthetic vs. biofertilizers) and different hydroponic system types, such as deep-water culture, etc. will be evaluated by identifying methylated and non-methylated regions in the whole genome sequence of the plants, such as lettuce and tomato through a bioinformatics approach. A functional profile of the bacterial species responsible for nutrient recovery in mineralization will be generated using metagenomics and meta-transcriptomics tools. Phylogenetic distribution will be identified for the top abundant aerobic/anaerobic bacterial species responsible for the mineralization of the nutrients. Various changes in top 10 aerobic/anaerobic bacterial species for 2 types of media will be studied through metagenomics, functional annotation by identifying various genes to explore mechanisms of adaptation, identifying various biological pathways responsible, changes in protein/proteome responsible for stress response, antibiotic resistance, changes in metabolism, post-translational modifications, etc.