Genome editing technologies hold transformative potential for aquaculture by enabling precise and efficient modifications to key traits, thereby driving advancements in commercial-scale production. These technologies offer unique opportunities to enhance economically valuable characteristics, including sterility, growth rate, and thermal tolerance, which are crucial for addressing challenges in sustainable aquaculture. However, achieving large-scale adoption requires overcoming significant technical, regulatory, and societal barriers. Here, we present results demonstrating high-throughput microinjection delivered genome editing in tilapia, achieving over 95% editing efficiency in somatic cells across thousands of embryos per day. Additionally, we showcase methods to utilize genome editing for the mass production of sterile fish, directly addressing environmental, regulatory, and societal concerns about genetically engineered organisms. This work highlights recent progress, innovative strategies for scaling genome editing, and the critical role of interdisciplinary collaboration in unlocking its full potential, ultimately fostering a more sustainable and resilient aquaculture industry.