Glyphosate is a non-selective herbicide that works by preventing the synthesis of proteins in plants. Glyphosate exposure in aquatic organisms can cause oxidative stress, cell damage, developmental impacts, physiological and behavioral changes, plant death, reduced biodiversity, bioaccumulation, and synergetic effects. In this study, we assessed the effects of the relevant concentrations of glyphosate (100-300 ppb) on young Litopenaeus vannamei through biochemical endpoints. During 50 days of glyphosate exposure increasing doses of Alkaline Phosphatase Assay (ALP), peaks at 150-200 ppb, and subsequently falls. In contrast, the SOD activity reached a maximum between 250 and 300 ppb. The tissues damaging enzymes GOT and GPT activity was increase rises at concentrations of 250 and 300 ppb. CYP 450 1A1 activities also incresead at 250-300 ppb concentrations. Overall, there was increased levels of tissue damaging enzymes, antioxidant enzymes and defence enzymes . Hepatic tissues of Litopenaeus vannamei exposed to commercial herbicide formulations containing glyphosate exhibit altered enzyme activities, suggesting an adaptive and regulated response against herbicide toxicity...