The Tropical Aquaculture Laboratory (TAL), located in Ruskin, Florida, is a leading research and extension center operated by the University of Florida. Established in 1996, the TAL was created to support the growth of the ornamental aquaculture industry through collaboration with key partners, including the Hillsborough County Board of County Commissioners, the Florida Tropical Fish Farms Association, and the Florida Department of Agriculture and Consumer Services. Over the years, however, its focus has broadened from fish health and applied aquaculture production to include topics such as reproduction, aquatic ecology, physiology, management of non-native species, youth education, and restoration aquaculture. The TAL also operates a fully-equipped diagnostic laboratory that provides services to address various aquatic animal health issues. With a long-standing commitment to advancing Florida’s aquaculture industry, the TAL works to address challenges and foster new opportunities. More than 25 University of Florida faculty, staff, and students are actively involved in its operations, and the TAL is positioning itself for future growth. The laboratory’s strategic plan aims to expand its programs, reach new aquaculture sectors, and enhance its facilities and resources to better support Florida’s diverse and evolving aquaculture industry.