Water quality is paramount for fish health and growth, with specific needs varying between life stages. Adult catfish thrive in temperatures of 65-80°F and prefer low turbidity and dissolved solids to minimize stress and support metabolic functions. Juvenile catfish, being more susceptible to environmental changes, require stable temperatures and minimal turbidity to ensure proper development and high survival rates. Understanding physical water quality is crucial as it directly influences fish metabolism, oxygen availability, and overall health. High turbidity can reduce light penetration and affect feeding behavior, while significant temperature variations can stress fish, leading to immune suppression and increased disease susceptibility. This study involves systematic sampling and analysis of water quality from both adult and juvenile catfish ponds located in Delaware State University Aquaculture Research and Demonstration Facility earthen ponds. This facility does not discharge water but recirculates through retention ponds to allow any potential sediments to settle and water to be filtered through submerged grasses to remove excess nitrogen and phosphorus build-up. Well water is the primary source for the facility. The ponds used in this study had 70x60dimensions. We had a total of 1000 juvenile catfish in each earthen pond. Water quality parameters consisting of water temperature, Dissolved Oxygen (DO), pH, and salinity were recorded using a YSI 556 Multiprobe DSS. Preliminary findings indicated that both the juvenile and adult catfish are living in suitable conditions for their given life stages. Future research will examine the long-term effects of physical water quality variations on catfish growth performance and physiological responses, further contributing to sustainable aquaculture management strategies.