Black soldier fly is one of the insect species receiving increasing research on its use as alternative protein and lipid sources in aquatic feed. As a lipid source, black soldier fly oil can provide up to 50% lauric acid, which has been shown to function as a potent antimicrobial and is beneficial to fish growth and health. However, overfeeding this oil may also cause negative impacts due to the limited contents of other essential fatty acids required by targeted fish species. Thus, it is critical to understand the acceptance of this alternative lipid source when used in feed formulated for different species of fish. Our goal of this study was to determine the potential application of black soldier fly oil in feed for juvenile walleye (Sander vitreus), an economically and environmentally important fish species in the Midwest region. Specifically, we evaluated the growth, health, nutritional composition, and tolerance to temperature shock of walleye fed diets containing various levels of corn oil replaced by black soldier fly oil. The basic dietary lipids contained 8% corn oil and 6% menhaden oil. Corn oil was replaced by black soldier fly oil at levels of 0%, 25%, 50%, 75%, and 100%. The protein and carbohydrate levels were the same across all the test diets. The feeding trial was conducted on walleye with an average initial body weight of 7.4 ± 0.06 g (n=18). Fish were raised in a flow-through water system (21°C) with three replications for each dietary treatment. Based on the 8-week preliminary observation, there was no significant difference in the weight gain (%), specific growth rate (% body weight. day), and feed conversion ratio ((p>0.05). No significant difference in mortality was observed due to the different dietary treatments (p>0.05). These results indicate that black soldier fly oil did not impair walleye feeding and can completely replace corn oil under the current testing conditions without negatively impacting growth or feeding efficiency. A final conclusion will be made based on the overall growth performance, fish health, nutritional composition, and temperature tolerance when this feeding trial is concluded at the end of the 10-week feeding period. The outcome of this study will provide insight into understanding the potential application of black soldier fly oil in walleye feed.