Cryopreservation is an essential tool in the protection of genetic diversity of valuable species, but the current capabilities of cryopreservation are highly limited due to lack of standardization and poor accessibility to equipment. Using the axolotl (Ambystoma mexicanum) as a model, we show how 3-D printed open-hardware devices, which are standardizable and inexpensive, can be used to cryopreserve sperm for in-vitro fertilization. Using a previously developed protocol, sperm from multiple axolotls were collected using a 3-D printed axolotl positioning device (i.e., AxoLounge), cooled at a rate of 15-20 °C/min using a positional cooling platform device (i.e., CryoKit), thawed, and applied to eggs to produce offspring. In the coming work, these devices and other essential supplies will be incorporated into a comprehensive cryopreservation capability kit to collect and cryopreserve axolotl sperm. This kit can be used in laboratories possessing only basic supplies and equipment to work with valuable biomedical or aquaculture species. In addition, such kits would allow multiple groups to contribute to the protection of imperiled species. A broad range of capability kits can be developed for aquatic species to increase accessibility to cryopreservation for new users interested in preserving genetic resources and provide much-needed standardization.