Sunshine Bass are hybrids produced by crossing White Bass females and Striped Bass males. These bass hybrids are an important food fish in the United States. Hybrids show heterosis for survival, growth, disease resistance and tolerance of environmental stressors. Production time for hybrids to reach a marketable weight of 0.6 kg-1.0 kg can range between 10-20 months. During this time, females can become sexually mature and produce eggs. When stressed these females can release eggs which in turn can adversely affect water quality. It is also thought that feed energy is directed toward egg production relative to muscle growth. As with many commercially produced aquaculture species, controlling reproduction can be important to optimize growth and energy allocation efficiency in this production cycle. Triploidization is an effective method to produce sterile fishes. This project aims to evaluate triploidy induction methods that have been used successfully with other commercial aquaculture species. Our goal is determining an optimum methodology for triploid production with special attention being placed on maximizing embryo survival and obtaining high triploidy induction in Sunshine Bass. Hydrostatic pressure shocks were evaluated to produce triploid Sunshine Bass embryos. Four pressures (5000, 6000, 7000, and 8000 PSI) and shock durations of 1.5, 2, 3, 4, and 5 minutes were evaluated. All shocks were administered 4 minutes post-fertilization. Thermal shock treatments were evaluated when administered at 3 or 4 minutes post-fertilization. Cold shock treatments of 2°C or 4°C were given for 10 or 15 minute durations and heat shock treatments of 36°C or 38°C, given for 2 or 3 minute durations. The non-shocked controls were also hatched. The fry produced were evaluated for triploidy using flow cytometry. The treatments yielded very low survival and triploidy percentages. Initially the flow cytometry results indicated heat shock treatments may have higher percentages of triploid induction, relative to cold or pressure shocks. More trials are required to fine tune the triploidization procedure for Sunshine Bass. During the upcoming spawning season, we will evaluate electrical shock as a way of producing triploids, and we will use the Coulter Counter to confirm our triploidy results.