Existing sensitive technologies for pathogen detection in aquaculture are slow and costly for large-scale deployment, presenting challenges to on-site decision making for pathogen control for preventing disease outbreaks. In this presentation, we discuss the development and performance testing of a novel acoustic biosensor for detecting pathogens in biological samples. The biosensor operation is based on immunoassay principles where capture bioprobes are designed to target pathogens via complementary binding, which is measured as an acoustic signal. Detection can be performed without requiring complex instrument or skills training for the performing personnel. The biosensor was tested for the detection of Vibrio parahaemolyticus in aqueous samples. Validation using plasmid-spiked samples showed high sensitivity, with detection ranges of 100-5000 gene copies/mL. These preliminary results demonstrate the potential of this biosensor as a rapid, efficient tool for pathogen monitoring in aquaculture. The biosensor’s modular design has the flexibility for detection of other pathogens by replacement of target-specific capture bioprobes.