Dietary grain sources can impact nutritional status, economic efficiency, and environmental impacts of aquatic animals. This study evaluated the feed quality and growth performance of rainbow trout fed extruded diets formulated with various sorghum grains and wheat. Four practical-type diets were formulated to be isonitrogenous and isolipidic at 45% protein and 18% lipid using wheat (GW), non-waxy red sorghum (NRS), waxy red sorghum (WRS), or waxy white sorghum (WWS) as the primary carbohydrate source. Diets were extruded using a Wenger X-20 single screw extruder and fed to rainbow trout (mean initial weight ± SD, 48.3 ± 0.70 g; 15 fish per tank) over a 14-week growth trial in a recirculating system at 15C with five replicate 400L tanks per diet. Feed manufacturing energy consumption varied due to grain choice, with GW requiring the highest total energy (532kJ/kg) and NRS the lowest (442 kJ/kg). Water stability and pellet durability (PDI) were significantly altered by grain choice; water stability was lowest in WRS (86.6%) and highest in GW (87.8%). PDI was highest in WRS (93.4%) and lowest in GW (86.0%). GW and NRS showed superior floating ability (100%), with WRS having the lowest (53.5%). Feed conversion ratios (FCR) were significantly affected by grain choice; WRS-fed fish had the highest feed intake (155.8%bw/d) and FCR (0.96). GW and NRS-fed fish exhibited lower feed intake (~140%bw/d) and improved FCR values (0.86 and 0.88, respectively). Visceral index and filet ratio also showed significant differences suggesting the need for further investigation to define the physiolocial implications of dietary grain type on nutrient utilization by rainbow trout. Overall, the results suggest that sorghum grains, both waxy and non-waxy, offer promising alternatives to wheat in aquafeeds, supporting rainbow trout growth with improved levels of pellet durability and acceptable water stability.