Plant-based ingredients are a cost-effective solution or replacement of animal protein sources in shrimp feeds, however, the presence of antinutritional factors (ANF) like non-starch polysaccharides (NSP) and phytate limit their inclusion. Phytic acid or phytate is commonly found in plant-based ingredients such as wheat, cottonseed meal, soybean meal, maize. When phytate binds to phosphorus (P), it makes it unavailable for monogastric animals, thus, the exogenous inclusion of phytases in the diets aids to increase the digestibility. Additionally, NSP are known to increase gut viscosity which decreases the rate of diffusion of substrates, preventing endogenous enzymes from breaking down feed molecules. The inclusion of exogenous enzymes like xylanase enhances the degradation of NSP into simpler sugars which are more readily available. Therefore, the aim of this study is to evaluate the effect and interactions between xylanase, phytase and a protease complex on the growth performance of Pacific white shrimp. Shrimp (0.23 g ± 0.0083) were stocked at 38 shrimp/m3 density in a thirty-two-polyethylene tank (805 L) recirculating system using green brackish water. Eight diets, including basal (no enzymatic addition) were formulated to contain 35% and 8% protein and total lipids, respectively. Three diets were made containing an inclusion of each enzyme, two containing the variation of two and one containing all the enzymes. Shrimp were fed using the handfeeding technique for 8 weeks. Growth performance parameters (survival rate, Biomass gain, feed conversion ratio, final average individual weight) were analyzed, although no statistical differences were evidenced (p>0.05). Subsequently, ten shrimp per replicate were analyzed for whole-body proximate composition, and protein and phosphorus retention were determined. However, there were no statistical differences among treatments (p>0.05). Gut and hepatopancreas tissues were collected, the results of which will be shared in the presentation.