High amounts of fiber such as non-starch polysaccharides (NSPs), and other antinutritional factors can limit the inclusion of plant-based ingredients in feeds. Complex polysaccharides like NSPs are broken down into simple sugars by carbohydrases, which can be more readily available for absorption. Endo-1,4-β-xylanase is widely utilized in nonruminant nutrition since it degrades Xylan, which is the second most abundant type of polysaccharide in nature into xylose. This enzyme has been reported to enhance nutrient utilization by improving digestibility and breaking down NSP in shrimp fed plant-based protein source diets. Xylanase is not endogenously produced; thus, an exogenous supplementation reduces the digesta viscosity in the gut allowing for a better nutrient diffusion, and an improvement in nutrient utilization. The aim of the research was to determine the effect of different levels of dietary Xylanase on shrimp growth. An 8-week trial was performed in a sixteen-polyethylene tank (805 L) recirculating system with brackish green water. Four levels of xylanase (ECONASE XT25, AB vista., US) were utilized (0, 0.05, 0.1, and 0.2 gr kg-1 of feed). Shrimp were stocked at a density of 38 shrimp/m3, and an initial weight of 0.20±0.0061 g (mean ± standard deviation). Shrimp were fed four times per day using the handfeeding technique. The feed amount was adjusted on a weekly basis. Growth performance parameters (survival rate, final biomass, final average weight, feed conversion ratio) were analyzed, and we found no statistical differences among treatments (p>0.05). Additionally, whole-body composition, protein, and phosphorus retention were determined. However, treatments were not statistically different (p>0.05). Gut tissue and hepatopancreas were collected, the results of which will be shared in the presentation.