This report seeks to validate the sea moss harvesting sites suggested in the draft “Irish Moss Harvesting and Aquaculture Guidelines 2021” document. The baseline assessment will explore the different types of sea moss found naturally growing in the benthic environment along Jamaica’s coastline. Knowledge of Jamaica’s wild sea moss population is important for exploring economic potential, supporting sustainable livelihoods, and community empowerment. Historical information was used to identify the primary locations islandwide where wild-caught sea moss is harvested. This was done by working with the NFA Extension Officers in each parish. A total of twenty-three (23) locations were identified within the coastal waters around the island. A visual survey method, Bohnsack, was used to assess the different bottom types and estimate the benthic percentage cover of sea grass, sand, mud, pavement, rubble, sea moss, and macroalgae in each area. A YSI 85 Oxygen Conductivity Salinity & Temperature Meter was used to collect parameters such as Salinity (ppt), Temperature (OC), Dissolved Oxygen in percentage (%), and Conductivity S/m) were taken at each site location. From the assessment it was found that a high percentage cover of sea moss exists in Whitehorses and Bowden Bay, St. Thomas; Manchioneal, Portland; Discovery Bay, St. Ann; Big Bridge, Westmoreland; and Alligator Pond, St. Elizabeth. The wild sea moss species found in Jamaica are: Gracilaria crassissima, Gracilaria debilis, GT Gracilaria and Kappaphycus alvarezii also known as (Eucheuma cottonii).