Latin American & Caribbean Aquaculture 2024

September 24 - 27, 2024

Medellín, Colombia

DETERMINATION THE CONCENTRATION AND INHIBITORY CAPACITY OF

POLYPHENOLS IN EXTRACTS OF Randia echinocarpa AGAINST Vibrio parahaemolyticus

Lugo-Gamboa Refugio Riquelmer*, Cervantes-Berrelleza Luis Jesús, Fierro-Coronado Jesús Arturo, García-Marciano Máximo, Apún-Molina Juan Pablo, Santamaría-Miranda Apolinar.

*Instituto Politécnico Nacional-CIIDIR Sinaloa, Blvd Juan de Dios Bátiz Paredes 250, San Juachín 81101, Guasave Sinaloa, México.

E-mail: rlugog2115@alumno.ipn.mx

 



The use of medicinal plants is proposed in the treatment of diseases that impact aquaculture because they synthesize and accumulate physiologically active substances that promote pathogen inhibition (antimicrobial, antioxidants, etc.). For this reason, the goal will be to evaluate the concentration and inhibitory capacity of polyphenols in extracts of Randia echinocarpa.

Drying of ripe fruit (RF) and leaf (L) was evaluated by freeze-drying, oven at 45°C and 75°C, and phenolic compounds and antibacterial capacity were determined.

The leaves had higher concentrations of total phenols in the three types of drying and in the three seasons where it is present, with higher concentrations in autumn with drying at 45°C.

The best inhibition extracted with 20% alcohol of R. echinocarpa extracts was found in the alcoholic pulp extracts (Conc. 100) with 77.13% against 6.56X108 CFU/mL of V. parahaemolyticus, while in the leaf extracts it was found at a concentration of 12.5 with 6.27% against 2.6X109 CFU/mL of V. parahaemolyticus, while the commercial antibiotic with 100%.

Conclusions: The most efficient drying to preserve polyphenols is in an oven at 45ºC. The best inhibition of Vibrio parahaemolyticus was found in alcoholic extracts of Randia echinocarpa pulp, with an inhibition of 77.13%. R. echinocarpa has potential as a source of bioactive compounds for the treatment of diseases in aquaculture, its effectiveness, especially compared to commercial antibiotics, varies depending on the part of the plant and the drying method used.