Introduction. Active surveillance of shrimp diseases involves sending tissue samples, such as muscle, pleopods, cephalothorax from a hatchery and grow-out pond to a diagnostic laboratory for disease screening using molecular biology and histopathology techniques. A comprehensive health assessment was made using H&E histology analysis of samples collected from major shrimp farming region in the country. Objective. The purpose of the study was to describe the histopathological lesions in shrimp, Penaeus vannamei collected during an active surveillance program of the ICA in 2023. Materials and methods. A total of 81 shrimp from the ICA active surveillance program of shrimp were sent to the LNDV for evaluation using routine histopathological techniques. These samples were collected from Covado island near from Cartagena, Bolivar, Colombia. For each shrimp, cephalothorax was always processed and only when gross lesions were identified also abdominal segment and muscle were included, sectioned, and stained with H&E staining at the LNDV following a standard procedure. The lesions were classified based on affected organ/ tissue, the characteristics of the lesion and the presence of the microorganisms. Results: Out of 81 shrimp examined using routine H&E histology, 50 shrimp (64%) showed integumentary cuticle disruption with Zoothamnium sp.; 46 shrimp (37%) showed gill cuticle with Zoothamnium sp.; 16 shrimp (13%) showed hepatopancreatic necrotizing granulomas compatible with vibriosis; 9 shrimp (7%) showed hepatopancreatic cells with intranuclear viral inclusions; 3 shrimp (3%) showed inflammatory muscle lesions with intralesional fungi categorized as infection with Microsporidium sp.; and, finally, 19 shrimp (23%) that did not present any microscopic lesions. None of the shrimp analyzed presented lesions characteristics of TSV, YHD, WSSV, AHPND, NHP and EHP. Conclusion: The present study highlights the potential of routine histopathology in diagnosing shrimp diseases in active surveillance program in shrimp aquaculture in Colombia. Among shrimp samples (N=81) examined by H&E histology, for 77% samples (N=62) this diagnostic test provided valuable information concerning to pathological status of the samples. The diseases putatively identified associated with corresponding pathologies are known to cause losses in shrimp aquaculture.