Galaxias maculatus is a small fish with diadromous and landlocked populations that plays an important ecological and socioeconomic role in Chile. This study determinate the sex ratio, sexual maturity stages, gonadosomatic index, spawning period, type and frequency of spawning, and fecundity under experimental culture. The sex ratio was close to 1:1 (female:male). First sexual maturity was reached at one year of age in 50% of the population. The highest GSI in both females and males was found in August (Figure 1). Nevertheless, females have a long spawning period divided into two sub-periods, the highest reproductive peak occurs between September and October and the minor peak occurs between December and February (Figure 2). Females spawn between three and 10 times over a period of two months. The number of embryos per female per day varied from 1 to 429, while the total number of embryos per female during the entire season varied from 163 to 1044. In conclusion, this species can be cultured successfully in captivity without compromising the reproductive cycle. This knowledge will be useful to establish future reproductive programs in captivity as a strategy for sustainable fishery and aquaculture management.