The current study was carried out for 147 days to assess the pathogenic Vibrio count of Litopenaeus vannamei culture ponds and the influence of probiotics from Mophus Bander, Srikakulam District, Andhra Pradesh, India. Three ponds were selected, one is control and other two were experimental (Pond A and B). The physico-chemical parameters of the culture ponds were recorded by adopting standard methods. During summer crop the control ponds were harvested at 24.0 gm on 107th day and at 31.0 gm on 121st day for the year 2022 and 2023 respectively due to the incidence of Vibriosis disease. Where as in winter crop during 2023 the control pond was harvested at 5.5 gm on 47th day because of Vibriosis but in 2023 the control pond was harvested normally at 32.5 gm on 145th day, as this pond is free of Vibriosis. Experimental ponds were harvested normally in summer season at 30.5 gm on 124th day & 31.0 gm on 127th day during 2022 and 34.0 gm on 127th day & 35.5 gm on 127th day in the year 2023 respectively.
Where as in winter season of 2022 the experimental ponds were harvested normally at 28.0 gm on 124th day as well as on 127th day. In the year 2023 winter, the experimental ponds were harvested at 36.0 gm at 146th day and 35.5 gm at 147th day respectively. This study suggests that the probiotics are the key agents which have a great impact on the reduction of total pathogenic Vibrio count in culture ponds of Litopenaeus vannamei.
Key words: Litopenaeus vannamei, Vibrio, Probiotics, sediment and temperature.