Eugenol has demonstrated a diverse array of beneficial properties, underscoring its importance as a bioactive phytoconstituent with a range of health-promoting effects, such as antioxidant, anti-inflammatory, and antimicrobial activity against a variety of pathogens, including Gram-positive and Gram-negative bacteria. In shrimp industry, acute hepatopancreas necrosis disease (AHPND) is the main bacterial disease which infected both hatchery and grow out pond. Eugenol self-assembled nanoparticle (E-SAN) was produced by heating in 120 ° C and proceed with solvent injection method. The size of particles is around 60-100 nm and MIC50 against Vibrio parahaemolyticus is around 40-80 µg/mL. The E-SAN has been proven no toxic effect based on MTT assay in hemocyte at concentration 400 µ g/mL and has antioxidant activity that able to inhibit ROS when hemocyte was induced with bacterin. The acute toxicity assay was tested in several dosages of E-SAN 40, 400, and 4000 µg/g to the shrimp for 96 hours. There was no mortality occurred during observation and also no significant different in histology of hepatopancreas. Moreover, as an alternative to replace antibiotic, E-SAN can be used as curative agent to infected-AHPND shrimp at 400 µg/g in dose by administrated orally. Further research needs to explore whether E-SAN can be used as an additive feed to protect the shrimp from AHPND infection.