Crustacean behaviour research has advanced in the last decades, with research focused on lobsters and crabs paving the way. However, the behaviour of the bulk of farmed crustaceans, i.e., Penaeid species, is poorly understood . Additionally, for shrimp species such as Penaeus monodon, there is still heavy reliance on wild-caught broodstock for seedstock production in hatcheries, and for all shrimp species, eyestalk ablation is still commonly employed by industry to induce satisfactory reproductive outcomes yet is attracting growing welfare concern . Understanding the behavioural profiles of P. monodon facilitates the assessment of the welfare impacts of husbandry interventions and strategies that may mitigate these impacts. The experimental system consisted of large aquaria with sand substrates, each equipped with individual custom computer modules to capture HD infrared recordings . Individual broodstock shrimp (N=16 for baseline behaviours) were stocked in sand bottom tanks for 72 hours. An ethogram was developed based on previously defined behaviours for other Penaeid species and refined with the identified behaviours in P. monodon, including walking, swimming, digging, feeding, grooming, eye beats, antennal scale flexion, forward antennal position, spawning and inactivity. Video analysis was quantified using Behavioural Observation Research Interactive Software (BORIS v8.22.6). The ethogram was then used to assess the response to husbandry interventions including ablation (N=8), ablation with topical anaesthetic (Lidocaine hydrochloride 20mg/g , N=8), handling (N=8), undisturbed (N=8), and a pleopod biopsy (N=8). These findings will contribute to our understanding of the impact of routine husbandry practices on the behaviour of broodstock P. monodon and provide a methodology for assessing welfare to be applied in future research on strategies to optimise broodstock management.