AQUA 2024

August 26 - 30, 2024

Copenhagen, Denmark

FEEDING BEHAVIOUR OF INDIVIDUAL BROODSTOCK Penaeus monodon: OPTIMISING FEEDING AND NUTRITION FOR REPRODUCTION

Artur Rombenso *,  Sarah Berry,  Emily Flanagan,  Isaak Kadel, Nick Wade , Caroline Lee, Greg Coman , and Dana  L. M. Campbell

CSIRO Agriculture & Food

Bribie Island Research Centre

144 North Street, Woorim, QLD, 4507 - Australia

artur.rombenso@csiro.au

 



 The advancement of prawn nutrition research, jointly with breeding and production systems, has supported th e expansion of  the global prawn industry .  Differently from larval and growout prawn nutrition, broodstock nutrition  still displays many gaps, including feeding behaviour and potential impacts on reproduction and ovarian development.  Accordingly,  our research group has developed experimental systems to assess the behaviours of individual broodstock P. monodon  to further understand basic husbandry practices including feeding.  The experimental system  consisted of eight aquaria with sand substrates, each  equipped with custom computer modules . Individual broodstock prawns (N=60 for feeding and feed preference) were stocked in sand bottom tanks for 72 hours. Animals were fed twice a day fresh-frozen squid and polychaetes. Behavioural responses to different feeds were described and categorised using Behavioural Observation Research Interactive Software (BORIS v8.22.6), and a previously established ethogram  for P. monodon was utilised, including walking, swimming, digging, feeding, grooming, eye beats, antennal scale flexion and antennae positioning. Time budgets and diurnal behavioural patterns were also developed to determine further baseline information of individuals to contribute to our understanding of individual variability in experimental systems .  Feeding and pre-feed behaviours, were examined including recognition of  presence of food, time to feed, observation of amount consumed in a single event and the attractivity and preference of type of food offered. T hese findings establish  a baseline understanding of individual broodstock prawn  feeding  behaviour and  provide  insights to  future research on strategies to optimise broodstock  feeding management to contribute to more sustainable methods of reproduction and seed supply globally .